2026-10-21T23:29:33Zhttps://adakent-rdlopen.rawdatalibrary.net/oai/request

oai:adakent-rdlopen.rawdatalibrary.net:adakent/100832026-10-21T19:35:05Zopenairedrivercom_adakent_10000col_adakent_10001com_adakent_10007col_adakent_10030

Ameliorative effect of Silk Fibroin against 5-Fluorouracil (5-FU)-induced gastrointestinal damage in ratsSexirli, Ahmet OzerAykaç, AsliÖzkayalar, HanifeSavtekin, GokgeSayiner, SerkanSilk Fibroin5-FluoruraciliNOSCD4+CD8+Objective: 5-Fluorouracil (5-FU), a widely administered anti-cancer drug, causes gastrointestinal damage through various mechanisms. This study aimed to investigate the ameliorative effect of silk fibroin (SF), which has anti-inflammatory and antioxidant properties, against 5 FU-induced gastrointestinal damage. Materials and methods: Wistar albino rats were divided into 3 groups; Control group, 5-FU group (30mg/kg/day on days 0, 2, 4), and 5-FU+SF (30 mg/kg/day 5-FU on days 0, 2, 4 + 600 mg/kg/day SF for 14 days). At the end of the experiment, stomach and intestinal tissue samples were collected and immunohistochemically iNOS, caspase-3, CD4+ and CD8+ were measured. Besides, structural damage was assessed with hematoxylin-eosin and caspase-3 and 9 activities were evaluated using western blot analysis. Results and Discussion: It was determined that iNOS, caspase-3, caspase-9 activities, CD4+ and CD8+ levels and structural damage significantly increased in both stomach and intestinal tissues. Furthermore, elevated markers were regressed almost to control levels in SF administrated group. Conclusion: SF may have an ameliorative effect, thus reducing the gastrointestinal damage caused by 5 FU. More clinical and scientific research should be conducted as the silk fibroin may be a suitable candidate against the adverse effects of chemotherapeuic agents.IBN SINA TRUST2023-10Articlehttps://rdlopen.rawdatalibrary.net/r/adakent/10083https://doi.org/10.3329/bjms.v22i4.686792223-4721enBANGLADESH JOURNAL OF MEDICAL SCIENCEinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://adakent-rdlopen.rawdatalibrary.net/tr/items/adakent/10083